Prototype Nutrition Ketoforce

Apr

11

Can Ketone Supplementation Cure Cancer?

By Patrick

 

Researchers at the University of South Florida recently had some very exciting research published in the International Journal of Cancer. http://www.ncbi.nlm.nih.gov/pubmed/24615175  The research team was led by Dr. Dominic D’agostino, and the goal of the study was to examine the impact of supplemental ketones and ketone precursors on metastatic cancer in mice.  The research has important implications on the treatment of cancer and also possibly on the prevention of cancer.

 

Ketogenic Diet, Caloric Restiction, and Cancer

Recent research has strongly suggested that the ketogenic diet may prevent the spread of metastatic cancer (metastatic cancer is cancer that can spread from the original tumor site to other parts of the body).  This research was pioneered by Dr. Thomas Seyfried from Boston College.   Dr. Seyfried has used the ketogenic diet in conjunction with caloric restriction to successfully restrict metastasis, and in some cases actually shrink the size of tumors.

The concept is relatively simple.  Cancer cells can generally only use glucose to energize their growth, while normal cells have the metabolic flexibility to use fatty acids and ketones in addition to glucose.  So by starving tumors of glucose via calorie and/or carbohydrate restriction – while simultaneously providing abundant alternative fuels for the body and brain (the ketogenic diet) – you may halt the cancer without side effects.

The concept may be quite bold, but it is not new.  A Nobel Prize physicist named Otto Warburg first conceived of the general idea back in the 1920s.  Unfortunately, after the discovery of the structure and function of DNA in 1953, the popular view of cancer was that it was a disease of purely genetic origin.  As a consequence, Warburg’s hypotheses were largely discarded.   Hopes were high that a cure for cancer was just around the corner back then, but as we all know that was not to be.   Luckily, within the last few decades some researchers have taken a second look at Warburg’s work and the “metabolic theory of cancer”.*

Using this “metabolic theory of cancer” as an inspiration, studies were undertaken examining the efficacy of caloric restriction and/or ketogenic diet in cancer survival.   It was found that often the growth and spread of tumors was halted, and in some case actually reversed.  These findings have garnered attention and have sparked some controversy. There are concerns with the practical applications of these dietary interventions in the patient population.  One concern involves  the idea of initiating caloric restriction while the threat of cachexia looms.   Cachexia is the condition of lean tissue wasting that often occurs as a consequence of cancer, and it is cachexia which often kills the patient rather than the tumors themselves.  Could caloric restriction exacerbate cachexia in some instances and lead to an earlier demise?  In addition to the cachexia issue, how readily will patients be willing to endure the restrictive and often unappetizing aspects of the ketogenic diet?

 

Ketone Supplementation

What Dr. D’agostino and his team set out to find is whether or not metastatic cancer could be controlled via exogenous supplementation alone.  He designed an experiment in which three groups of mice were inoculated with a highly metatstatic line of cancer cells that contained a luminescent tag (to enable clear imaging of the tumors).  All the mice were given unrestricted access to a standard diet (60% carbohydrates).  One group would have the diet alone while the other two groups would receive one of two different forms of exogenous ketone compounds in addition to the diet.  The two exogenous ketone supplements used were 1,3-butanediol (BD) and RS-butanediol diacetoacetate (ketone ester or KE).  Their metabolic conversions to ketone bodies is exemplified in the following diagram

 

ketone-diester-metabolism

 

The results of this experiment were remarkable.  Despite being on a high carb unrestricted diet the groups taking the exogenous ketones exhibited dramatically less tumor infiltration throughout the body compared to the diet alone mice (as seen by bioluminescent imaging).  Survival in the BD supplemented and KE groups were prolonged 51% and 69% respectively compared to controls as well.

These results seem to contradict much of what was assumed regarding how the ketogenic diet fights cancer.  It has traditionally been thought  glucose deprivation was necessary to starve the cancer.  However in this study the mice were eating unrestricted amounts of a high carb diet, so glucose remained high.  What could be going on here?   The researchers speculate that the ketones themselves may be directly toxic to the tumor cells, via mechanisms such as disruption of energy production by glycolysis in the tumors.

 

Impact

This paper certainly will cause an upheaval amongst those studying dietary interventions for cancer.   Now the possibility has been opened up for simple supplementation as a course of treatment using the synthetic ketone precursors used in the study, or perhaps even by using natural ketone supplements such as beta-hydroxybutyrate (BHB) salts.   Supplementation would be a much simpler, convenient, and patient compliant alternative to the more drastic dietary interventions previously thought necessary.

 

 

 

 

*The metabolic theory of cancer postulates that cancer does not arise because of damage to our DNA resulting in mutated oncogenes, but rather it arises as a result to disruptions in processes of energy production in our mitochondria.  The disruption of oxidative phosphorylation in the mitochondria forces a cell to rely on the less efficient glucose driven anaerobic glycolysis.   It is this dysfunction in energy production which then is thought to turn on existing oncogenes in the nucleus, causing the cell to turn cancerous.

 

Dec

9

Espindolol (S-Pindolol, MT-102)

By Patrick

 

Anabolic drugs are not dead, in fact research to develop new ones are aggressively being pursued to address medical situations such as cancer cachexia (wasting secondary to cancer) and sarcopenia (age related loss of muscle mass).  Both of these categories are potentially huge money makers.   Amongst the weird drugs that are being proposed for these applications is one that kind of boggles my mind.  It is actually a current drug used to treat high blood pressure.  Well, I guess you can say HALF of the drug is being developed as an anabolic

The drug I am referring to is called Pindolol, and the “half” that is being developed is S-pindolol.   What I mean by that is that Pindolol is what is known as a racemic compound (it exists in a 50/50 ratio of right and left handed isomers) and the drug proposed as an anabolic is S-pindolol (the left handed isomer only).  Many drugs are like this and often only one isomer is the active one.  Take for example methamphetamine.  Its active isomer is the right handed one.  The left handed one is weak enough that it is allowed to be sold over the counter as a component of vicks inhalers.

Pindolol is sold to treat high blood pressure and it does so by blocking the effects of adrenaline like compounds on the heart (it’s a beta1 blocker).  As such it decreases the force and frequency of heartbeats and thusly blood pressure decreases.  Interestingly, pindolol also has agonist effects upon receptors of adrenaline like compounds too, specifically on skeletal muscle (it’s a beta2 agonist).  You may be familiar with beta2 agonists such as clenbuterol.  They are anabolic

Anyway, this compound (the S-isomer of pindolol which is the isomer that does stuff) is poised to be marketed as an anabolic for cancer cachexia and maybe sarcopenia.   Studies have been done that have demonstrated muscle growth in these models.  Myostatin has been shown to be suppressed amongst other things, which are effects we know happens with beta2 agonists (at least in the short term).  Cool thing is that unlike most beta2 agonists (clen) this has no stimulatory effect on the heart.  Not so cool thing is that it actually has a depressive effect on the heart, which means your exercise performance can suffer because your heart won’t beat fast and hard enough to support your efforts (that is if you train like a man)

Anyway, interesting stuff I suppose and expect it to appear on your banned list soon

 

Sep

26

DMAA: Not Dangerous After All?

By Patrick

 

DMAA, also known as methylhexanamine or “geranamine” is a controversial stimulant ingredient used in weight loss and energy supplements.  In April of this year the FDA made a strong statement regarding its health dangers and its lack of legal standing as a nutritional supplement.   Companies selling DMAA products for the most part stopped selling the stuff, and those who didn’t fully comply were subject to harsh enforcement actions by the FDA.

It took a long time for the FDA to act on DMAA, and that was in large part due to the fact that they really didn’t have clear cut evidence that the product was dangerous.  Outcries by certain people that claimed the product was responsible for various adverse medical events and deaths however became louder and louder.   These claims were not substantiated by any medical evidence though and so were not enough for the FDA to act upon.

The most publicized adverse health events regarding DMAA involved the US military.  The deaths of four servicemen were being blamed on DMAA.  As a consequence, the department of defense commissioned a safety study on the compound to determine whether it indeed was dangerous and to blame for the soldiers’ deaths (as well as other medical incidents involving soldiers).  This was to be the study that the FDA could rest its hat on and justify an emergency action against DMAA.

Things started getting strange though.  The study was supposed to be finished in February 2012.  That date came and went and no word on the study results were announced.  Then word came that the study was taking longer than expected and would be done in December 2012.  Well December 2012 came around and still no word.  Months passed and no one seemed to be talking about the huge DOD study that was supposed to prove once and for all that DMAA was deadly.

Then in April 2013 the FDA announced that it considered DMAA illegal to sell and warned of a whole variety of potential health risks.  No mention of the DOD study which was supposed to provide the scientific validation was made – the FDA announcement was based simply on theory.

What happened to the DOD study?   Well, in August the results were finally released (four months after the FDA’s arbitrary action).  They were released with such lack of fanfare and media coverage that even I was not aware of the results until just today (almost two months later).  Essentially they found that despite a high apparent usage of DMAA by soldiers (as much as 15 percent) the substance at doses recommended by manufacture poses a low risk of serious harm for most service members.  The study basically exonerated DMAA from being responsible for the deaths of the four soldiers.  They cautioned though that the “potential” of DMAA to cause harm still exists and ongoing studies would be needed to fully understand the health issues (I guess two years wasn’t enough).

Anyway, I have my own take on this situation.  I think it was clear quite a while ago that the DOD study was not going to provide the smoking gun that was expected and hoped for.   At that point the results were kept hush hush and the FDA decided to act anyway against DMAA.  Then they waited four months to quietly announce the study results – so quietly that it took me six weeks to even be aware of them.

http://www.naturalproductsinsider.com/news/2013/08/dmaa-cleared-in-army-deaths-still-poses-risks.aspx

 

Aug

12

Initial KetoForce (BHB mixed salts) Data

By Patrick

On my last blog I promised  I would show you some data on my KetoForce product (BHB mixed salts).  I have two sets of data – the first showing blood beta-hydroxybutyrate (BHB) responses and the second showing specific changes in physiological parameters during a controlled exercise experiment.

The blood data was done at a university on a set of subjects and the average changes in BHB levels are what this graph shows

 

bhb graph

 

These subjects drank a diluted version of the KetoForce product that was essentially equivalent to 36mL of KetoForce.*  The product was consumed on an empty stomach and as you can see blood BHB levels rose pretty quickly.  Levels appear to stay decently elevated until round the 180 minute (3 hour) mark.   30-60 minutes seems to be the sweet spot for maximum blood levels, so for pre-exercise use one should probably time the ingestion accordingly.

The exercise data I have was done using a 20% solution of the BHB salts which was equivalent to 40 mL of the  KetoForce product.  The data was done by an independent individual that does such research for a non-profit organization.  Out of respect for this fact – and the fact that the data is only preliminary (more research is going to be performed) – I won’t report the full data here.  I will give a glimpse into the most interesting thing he found though.

First of all this individual was ketoadapted.  Ketoadapated means he had been on a ketogenic diet for a period of time long enough so that he was in full ketosis and had developed a good ability to utilize ketones.  He did a bike exercise that was set up so the workload was maintained at a constant level roughly equal to 60% of his VO2 max.  During the first stage he did the exercise for twenty minutes without the salts, and during the second stage he consumed the salts 60 minutes prior to the exercise bout.

One of the things that was measured was the amount of oxygen consumed during the exercise bouts.  Theoretically, ketones should reduce oxygen consumption because they are known to generate more cellular energy per unit  oxygen burned compared to glucose and other energy sources.  The data he got with the BHB salts strongly suggested that this theory was at work during the experiment.  Below is the difference in oxygen consumption reported as a percentage between a bout without the salts and a bout with the salts.

Last 5 minutes -  minus 8.8%

Last 10 minutes – minus 7.7%

Last 15 minutes – minus 6.6%

Apparently there was a substantial decrease in the amount of oxygen he needed to maintain the workload after taking the salts.  Very interestingly, this decrease in oxygen demand got more prominent as the session went on as well.

BHB salts are natural products that mimic the nutritional state of ketosis and have been studied in the past for medical applications as well.   KetoForce is  the first introduction of these salts to supplement and fitness industry so we are very early on in regards to figuring out how best to use them.  This preliminary data does give us some clues on how they may be used (timing wise) and what benefits they may offer.  We are very hopeful that our collaborations will enable us to generate a lot more research on the product (such as what performance benefits might be seen in non ketoadapted people)  in the very near future.

 

https://www.prototypenutrition.com/ProductDetails.asp?ProductCode=KF

 

*  The KetoForce label suggests a serving size of 30mL instead of 36mL.  Since I don’t expect all my customers to own graduated cylinders many will  have to use the cap for measuring, and the cap holds 10mL .  For this reason I rounded the serving size off to 30mL.

Aug

2

Instant Ketosis?

By Patrick

 

In late 2010 a professor from a university in Florida approached me to see if I could synthesize a compound that he wanted to use in his research.  This compound was what is known as a “ketone ester”.  This ketone ester is a synthetic prodrug which, after ingestion, your body naturally breaks down into the ketone bodies beta-hydroxybutyrate (BHB) and acetoacetate (AcAc).

Ketone bodies are energy sources that your body naturally produce and burn under special metabolic conditions.  These conditions include starvation and a very high fat / low carbohydrate diet (known as the ketogenic diet).   Ketones are the end products of fatty acid metabolism, and compared to your body’s major source of caloric energy (glucose), they are pretty amazing.  Unlike glucose, ketones do not require insulin to enter cells and be incorporated into the metabolic cycles that generate the cellular fuel ATP.  They cannot be converted to body fat. Also, unlike most fats, ketones can freely enter the brain where they are an excellent fuel for brain cells.  On top of all that, ketones generate more ATP per unit oxygen consumed than any other energy source.  Ketones are essentially your body’s super fuel.

I ended up successfully synthesizing this ketone ester for the professor, and he ended up performing some pretty cool studies utilizing the product.  For instance in one study he made rats resistant to the toxic effects of high pressure oxygen upon the central nervous system (this has major implications for deep sea divers such as navy seals, as well as indirectly on folks suffering from epilepsy).  In another study he almost completely halted the growth of cancerous tumors in rats by giving them the ketone ester.  The professor also showed in a more informal study that rats taking the ketone ester could exercise quite a bit longer than control rats.

As a supplement manufacturer I found the potential health and performance implications for raising ketones through the administration of a “ketone supplement” very exciting.  Unfortunately the ketone ester I made for the professor is both  synthetic and extremely expensive, so it was wholly unsuitable for sale as a supplement.  My collaboration with the professor did get me thinking though, and I set out to see if I could develop a way to raise ketone levels by administering a natural product alternative.

My focus was on the ketones themselves.  As I mentioned previously, there are two major endogenous ketones: beta-hydroxybutyrate (BHB) and acetoacetate (AcAc).  AcAc I had to eliminate right off the bat because it is too unstable (it breaks down relatively quickly to acetone and carbon dioxide).  BHB on the other hand was a possibility – particularly in the form of one or more of its salts.  The problem was the only BHB salt which is commercially available is sodium BHB and it’s just too expensive.  So I had to embark on my own R&D project to figure out how to make BHB salts at a price that is not completely out of reach.

I ended up perfecting a proprietary manufacturing method for sodium and potassium mixed BHB salts.  Why mixed sodium and potassium salts?  The reason for that is because at the level of intake of BHB required for benefits you would have to ingest a substantial amount of cation (positively charged mineral such as sodium or potassium).  At such levels of cation ingestion it made sense that a balance of sodium and potassium salts would be the healthiest thing to do.

Additionally, I discovered that the product had to be delivered as a concentrated liquid because potassium BHB is simply too hygroscopic to isolate as a powder (it picks up water from the atmosphere and turns into mush at an astonishing rate, whether by itself or mixed with sodium BHB).

So, just to make things clear, is I have developed a supplement product that raises blood ketone levels.  This product does not require a ketogenic diet to raise ketone levels as it is essentially providing an external source of ketones.  As such, it will raise ketones even if you eat a plate of spaghetti before taking it (still, its benefits are maximized when combined with either a ketogenic or other sort of low carb diet).

This kind of thing is a lot of work.  For instance it involved developing novel analytical testing methodologies (which as an organic synthesis guy isn’t second nature by any means!)  But after about a year of work  this weekend I am proud to announce the introduction my sodium and potassium mixed  BHB salts on my prototypenutrition.com website.

https://www.prototypenutrition.com/ProductDetails.asp?ProductCode=KF

I hope to continue this topic next week and introduce you to some blood data and exercise performance data on my BHB salts that I think you will find very exciting.  Also I will give some tips on how to possibly best uitilize this product for whatever health or fitness goals you may have.

So stay tuned!

Jun

4

Progesterone Derivative Sold as Prohormone

By Patrick

 

I was recently asked a question about a very strange new prohormone product.  What makes it strange is that is actually based on a female hormone – specifically it is based upon a hormone called 17alpha-hydroxyprogesterone.

17a-hydroxyprogesterone (or 17-HP) is a hormone intermediate in the steroidogenic pathway between progesterone and androstenedione.  17-HP is present in the blood of women in varying amounts during their monthly cycle and is present in particularly high amounts during pregnancy.  It has similar actions to progesterone  (albeit somewhat weaker), and is thought to serve a complimentary role to progesterone as an endogenous progestogen.

The prohormone in question here is not actually 17-HP however, but a close structural derivative to 17-HP that I will call Dehydro 17-HP.  For all intents and purposes however, the metabolism of the derivative should be analogous to 17-HP.  Specifically, here are the potential pathways of the two

17-HP ——– Androstenedione  —– Testosterone

Dehydro 17-HP ——— Boldione ——–  Boldenone

 

So it is definite that the potential for 17-HP and Dehydro 17-HP to convert to active anabolic/androgenic hormones is there.  There are two questions though.  How much do they convert and is there any HPTA suppression from the progestational action of the parent compounds?

Perusing the research on 17-HP I did find a little bit of information on the first question.  Apparently at least one study found that given to humans and to rats the compound results in a substantial increase in urinary 17-ketosteroids (androgenic metabolites).  This indicates that it does convert to a significant extent to androstenedione at least.  Furthermore, a study where 17-HP was given orally to cockerels (immature roosters) showed it to have one half the androgenic activity of methyltestosterone.  It is important however to note that androgenic activity in a cockerel is measured by the size of that red comb on their heads, which is not necessarily easily translatable to anything in a human.

The answer to the second question –  involving whether the compound is HPTA suppressive – is harder to answer.  Data on women show varying effects on 17-HP at different times throughout the cycle, but this data is irrelevant to men.  I couldn’t find anything on men and LH/FSH levels.  So that part of the equation remains unanswered.

I guess I don’t really know what to say about this stuff.  If it works at all I would expect one to have to take many hundreds of milligrams before an effect is seen (due to it being a two step conversion via an intermediary dione).  Whatever the case, it is sort of amusing how creative companies will get in an attempt to fulfill customers’ demands while trying to minimize legal exposure.

Apr

9

More Craziness over Craze

By Patrick

As I said in my last piece, things are moving fast in this Craze scandal.  Word just came out today that the product was tested in Sweden and confirmed to contain the same meth analog that the Australian federal police said they identified

“In this material we found substances chemically similar to amphetamine and that probably would have similar effects to amphetamine has been says Anna Stenfeldt Henning, Head, Drug Analysis Unit, SKL.”

SKL is the Swedish National Lab for Forensic Science. The Swedes refer to the chemical as N-ethyl-1-phenyl-2-butylamine, but it also goes by the names N,alpha-diethylbenzeneethanamine and N,alpha-diethylphenethylamine.  As I mentioned in my last installment it is considered a structural isomer of methamphetamine.  Why is it considered a structural isomer to methamphetamine while some other phenethylamine derivatives are not?  If you want to get technical it is because it contains an alkyl (hydrocarbon chain) alpha to the nitrogen atom.  The presence of the alkyl group blocks the enzyme monoamine oxidase, which normally inactivates such neurotransmitter type compounds.  In other words it makes it potent so you get the classic “speed” high.

This testing by the Swedes comes on the heels of a consumer who took Craze for two weeks and ended up in the hospital.  This case led the magazine SVT Nyheter to contact the SKL who then took it upon themselves to test the product.

The makers of Craze have responded to SVY Nyheter by claiming that their product does not contain the meth analog and that it must be one of the counterfeit Craze jars that allegedly have been floating around europe.  However, SVT Nyheter contacted the retailer that they bought the Craze from and they claimed to have bought it directly from Driven Sports.

I have yet to hear of any action regarding this product here in the states.  Online retailers continue to sell the product.  I don’t know if that is going to continue for long.   The liability of selling a product with the knowledge that it may contain an illegal and potentially dangerous substance might be too risky for large distributors and retailers.  Usually in situations such as this the product is put on temporary hold until more information is available and the situation clarified, because not to do so puts a company at potentially greater legal risk in the future should the fears be confirmed and a criminal investigation launched.

So if you love your Craze, and you don’t care about any of the scary stuff coming to light lately, you might want to stock up.

www.svt.se/nyheter/regionalt/vastnytt/vanligt-kosttilskott-innehaller-dopningsmedel

Apr

4

Craziness over Craze

By Patrick

Driven Sports (formerly Designer Supplements) is a somewhat popular company in the sports nutrition arena. They have a dedicated cult following due to their history of introducing controversial and intriguing products such as the now infamous designer steroid “superdrol” to the market place. Their latest hit product is a “pre-workout” product called Craze. This product gained insane popularity over the last couple of years and users who have taken it have reported amazing energy and euphoria. Since the ingredients on the label did not seem to list anything that could explain such remarkable effects, much suspicion arose over whether the product might be “spiked” with some sort of undisclosed stimulant compound.

Well, in early February that suspicion reached a fever pitch when supplement retailers disclosed that federal police in Australia told them that Craze (which has been a popular import there) was found to contain a methamphetamine analog. It appears from various sources that the analog they were referring to was N-alpha-diethyl-benzeneethanamine.

As a consequence, Craze has been banned from Australia and all imports are now being confiscated. Curiously, this comes right on the heels of an announcement of the ban of a Rugby player by the Australian Sports Ant-Doping Authority (ASADA) for the same chemical analog plus another analog called 1-phenylbutan-2-amine (which interestingly would be an expected metabolite of the analog allegedly found in Craze). No mention however is made that this positive is the result of the athlete ingesting Craze.

I am following this story closely and right now there is nothing completely confirmed by any official documentation. One thing is curious though, and that is the fact that craze does contain a compound on the label with the exact same molecular formula – differing only by the placement of one of the ethyl groups.

Very curious. What could this mean? Could the aussies have mistaken this listed ingredient for the meth analog? Very doubtful, as they must have been fully aware of the difference. Also, from my research the actual mass spectrum for the analog appears to be recorded in the literature, and so could be matched up. Conspiracy theorists might imagine that a company who wanted to slip such an analog into a product could try to throw testers off by listing an extremely close structural isomer on their label. Hmmm…

Right now it is not clear what is going on but things are unraveling and I will keep everyone updated soon. As far as what the ramifications for Driven Sports would be in the United States should this all prove to be true….well…. that can vary from minor to major. Perhaps a warning from the FDA or worse a criminal charge of adulteration / misbranding etc. Worse still would be a felony charge of distribution of a controlled substances, since analogs of CII substances like methamphetamine can be considered also controlled under US law.

Stay tuned

 

 

mass spec

Feb

5

Stem cell breakthrough for muscle repair and growth??

By Patrick

What if they could liposuction fat out of you, put it in some machine, and then get super cells out the other end that you could have injected into your muscles to repair injury and possibly cause local growth? It sounds pretty cool doesn’t it? Apparently this is what some new research suggests is possible, and thanks to some recent technological breakthroughs it appears that it might not be too difficult or expensive to do.

You probably have heard of stem cells. Stem cells are a special kind of cell that exist in a sort of “unprogrammed” state. There are many different kinds of stem cells that can be derived from many different tissues, but the fundamental property they all have is a varying ability to differentiate from their primordial state into cell types of all sorts such as blood cells, heart muscle cells, bone cells, organ cells, brain cells etc.

The most useful stem cells are those derived from embryos and they are known as “pluripotent” stem cells. That basically means they have the ability to differentiate into any cell type in the body. Due to ethical concerns, and the obvious raw material limitations of harvesting embryos, this sort of stem cell does not enjoy practical exploitation in medicine. There are also stem cells that can be harvested from adult tissues. Although adult bodies primarily consist of fully differentiated cells that serve our various needs, there also are stem cells present. Adult stem cells are known as multipotent stem cells. They don’t quite have the flexibility to easily morph into anything like embryonic stem cells do, but they can be coaxed to differentiate into lots of useful cell types. And of course they don’t share the controversy and limitations associated with embryonic stem cells.

Blood marrow has been one such important source of multipotent adult stem cells for medical research and application in the recent past. As useful as bone marrow is however, the process of drilling into your bone to suck out the marrow is invasive and distasteful. Thankfully, in recent years it has become apparent that fat stores harbor an impressive variety of adult stem cells as well (reportedly containing 100-300 times as many useful cells as blood marrow).

Fat stores are easily accessible. Cosmetic surgeons suck out pounds of the stuff every day from women’s hips and men’s bellies and then proceed to chuck it all in the medical waste bin. Adipose tissue derived regenerative cells (ADRCs) as they are called can be isolated from all this waste fat, however until recently the process has been less than rapid and practical because it involves a prolonged culturing period. Luckily, a new filtering system has been developed which rapidly separates out the ADRC fragments of the fat and dumps out all the needless junk. [ADRCs by the way consist partly of adipose tissue derived stem cells as well as other progenitor cells of value to injury repair such as vascular related endothelial cells, fibroblasts, and preadipocytes.]

In recent years there have been some interesting studies performed using ADRCs in muscle tissue. In one study, the cells were injected into mice with muscular dystrophy and the muscle strength and resistance to fatigue improved http://www.ncbi.nlm.nih.gov/pubmed/21874281. In another, the cells were injected into normal mice and new muscle tissue was observed to be formed at the injection site http://www.ncbi.nlm.nih.gov/pubmed/16887099. Just recently though a study was released looking at the effect of ADRCs on muscle tissue injury in mice, and the mechanisms of the actions were examined in more detail then in the previous studies http://www.ncbi.nlm.nih.gov/pubmed/23239611.

In this study rats had their tibialis anterior muscles lacerated with a scalpel. One group had a simple phosophate saline solution injected at the site, a second group had human bone marrow stem cells injected, while a third were injected with human ADRCs. The rats injected with ADRCs showed greatest improvement in healing out of the three groups. The formation of new blood vessels (angiogenesis) and new muscle cells (myogenesis) was observed, and fibrosis was suppressed.

The researchers looked to see exactly what was going on. Since the rats were injected with human progenitor cells they could examine certain markers to see if these cells were differentiating into muscle and vascular cells in the rats. Although that was observed to a small degree, it appeared that what was mostly going on was that these injected cells were secreting lots of cytokines and growth factors such as vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF), and these were stimulating the rapid repair and regrowth of the native tissue in the injection area.

Whatever the mechanism, the technique seems to have potential due to its ease of preparation and also due to the low risk of immune rejection (since one’s own cells are being reinjected into their body). Also, we don’t know whether if when human ADRCs are injected into human tissues there may be more actual differentiation into new tissue going on, and not just mostly paracrine stimulation of growth of existing tissue from secreted growth factors.

I expect to see this technique to soon gain popularity in elite sports injury repair along with current new fangled techniques such PRP injections.

Jan

11

The Bulimia Machine

By Patrick

You may have heard of the new personal stomach pump invention by the guy that brought us the Segway personal transport thingy. The contraption is called the AspireAssist Device. In Europe the device has been clinically tested on several overweight people and found to be very effective over the long term. 24 obese people lost an average of 49 percent of excess weight (which comes to around 45 pounds) after using it as directed for a year. The makers of the AspireAssist device hope to gain FDA approval sometime in the near future.

This story has caused a lot of controversy. First of all, the product itself works in a rather disgusting manner. Basically it works like this – you get an operation where a tube is installed the goes from the inside of your stomach out through the front of your body. There is an opening on your belly skin where you can stick some sort of pump device. The idea is, after you eat a meal and wait 20 minutes (to let your food digest and get liquified and evenly dispersed) you then use the pump to remove 30 percent of your stomach contents. [I have no idea how it is able to gauge what 30 percent of your stomach contents are, but then again I don’t know how a Segway works either.] You then spray these contents into a suitable disposal apparatus such as your toilet, or if you are an animal lover, a baby birds mouth.

So why is this controversial you ask? Well, where do I start? First of all, the idea grosses people out and reminds them of bulimia (bulimia is generally considered a very bad thing). It also is offensive to those people who believe one should approach dieting from a healthy direction – with lifestyle changes that involve healthy food choices and eating habits and all that stuff. To them the idea of a quick fix approach sounds abhorrent, especially one that just seems so creepy. Some folks also express fear that this device may lead to health issues associated with classic bulimia, such as nutritional deficiencies and metabolic dysfunctions.

I think everyone should just chill out. First of all, if this device is approved it surely will be only for morbidly obese folks, and then only after classic diet and behavior counseling has proven ineffective. Mobidly obese folk are very sick and they almost always have severe diabetes and accompanying health issues that I can’t even begin to list. Right now the options for these folks are not good and usually involve some sort of bariatric surgery. Bariatric surgery is a horrible procedure and carries a frightening fatality risk. Plus, it can be absolute torture to prepare for, and even worse to recover from. This AspireAssist device on the other hand requires a relatively simple surgical procedure to install, and if needed the device can be removed quite easily and rapidly as well. And what about the risk of nutritional deficiencies and associated bulimia type issues? I don’t see the possibility of that, since you are removing stomach contents which should be homogenously distributed (if you wait the required 20 minutes after eating), and you are only removing 30 percent of the contents. It’s not really different then if you just ate 30 percent less to begin with.

So once you get over the yuck factor and think about it, AspireAssist may actually prove to be a pretty nifty and valuable medical breakthrough. Technological advancements are often met with alarm and disdain, especially if they encroach on sensitive subjects such as personal appearance and fitness. In the end though, they can come to be accepted if they fulfill their promise.